Proteostasis and Proteomics of Aging Core
Alterations in proteostasis have been described in multiple age-related disorders and are also tightly linked to additional mechanisms of aging such as molecular damage, cellular response to stress, metabolism, inflammation and stem cell functioning. Genetic interventions in invertebrates and in mammals, now support beneficial effects in life- and health-span of modulating components of the intracellular pathways responsible for proteostasis maintenance, chaperones and the proteolytic systems.
Translation of these findings into humans requires:
- characterization of changes in proteostasis in old organisms;
- understanding the molecular mechanisms behind proteostasis loss in aging and
- extensive testing of the effect of chemical and natural compounds on the proteostasis machinery and their future development into drugs.
The Proteostasis of Aging Core (PAC) was created in response to these needs of the aging research community and, during its ten years in operation, has been providing reliable and highly validated services, state-of-the art methodology, expertise and cutting-edge knowledge related to proteostasis to internal and external E-NSC members and aging research groups worldwide.
Personnel
Director: Ana Maria Cuervo MD Ph.D. and Simone Sidoli, Ph.D.
Operations Manager: Antonio Diaz
Consultants: Dr. Evris Gavathiotis, Dr. Susmita Kaushik
Services
Assays
- Autophagy profiling
- LC3-II flux
- Protein turnover
- Electron microscopy and morphometry
- Lysosomal functions
- Enzymes assays
Reagents and Samples
- Autophagy antibody panel
- Autophagy reporters
- Lysosomal markers
Consulting
- Autophagy methods and assays (Dr. Susmita Kaushik)
- Drug developments and design (Dr. Evris Gavathiotis)
Prices
| Type of Service | NSC Member Rate | Others |
|---|---|---|
| Complete Protein Turnover analysis | $600 | $950 |
| Protein synthesis (4 samples with triplicates) | $200 | $300 |
| Protein degradation (1 type sample 6 conditions with triplicates) | $500 | $600 |
| Type of Service | NSC Member Rate | Others |
|---|---|---|
| Macroautophagy (MA) | ||
| LC3 flux - immunoblot (8 conditions) | $350 | $550 |
| mCherry-GFP-LC3 High content microscopy/96 well plate | $600 | $800 |
| Mitophagy - immunofluorescence colocalization/8 well | $300 | $400 |
| Lipophagy - immunofluorescence colocalization/8 well | $500 | $750 |
| Em morphometric analysis of autophagic compartments*** | $200 | $300 |
| Type of Service | NSC Member Rate | Others |
|---|---|---|
| KFERQ-Dendra - High content microscopy/96 well plate | $600 | $800 |
| Lysomal Function | ||
| Lysosomal compartment – Lysotracker/ 8 well | $250 | $250 |
| Lysosomal markers (panel) - Immunoblot | $300 | $600 |
| Type of Service | NSC Member Rate | Others |
|---|---|---|
| Degron reporter – High content microscopy/ 96 –well plate | $650 | $950 |
| Consultation | ||
| Consultation on study design, methods, interpretation | $95/hr | $200/hr |
| Training in proteostasis analysis procedures | $50/hr | $-- |
| Other Services | ||
| Customized analysis of proteostasis | Negotiable | Negotiable |