Health Span Intervention Core
There are clear phenotypic changes that occur with aging, including shifts in energetics and metabolism, behavioral and functional deficits, and these phenotypic changes are accompanied by increased pathologic lesions and burden in tissues with advancing age. These effects can further be mediated by cell autonomous and cell non-autonomous mechanisms, which are interrogated via strategies, such as heterochronic parabiosis. To support demands for these services to the research community, the HSC was established as a critical resource within the E-NSC to perform integrative phenotyping of health and function across multiple domains in aging rodents by leveraging our long-standing expertise in conducting sophisticated in vivo metabolic studies, hormonal assays, and specialized surgeries, including parabiosis, as well as newly added services, including geropathology, behavior, and cardiovascular function. These studies are supported by an experienced and skilled team of staff scientists and technicians, and a distinguished group of expert consultants. The HSC also offers consultation, advice, and training to investigators interested in implementing these studies in their own lab, or to devise a customized study design for services in the Core. The HSC also makes an aging and parabiotic tissue repository available for dissemination to investigators upon request.
Personnel
Director: Derek M. Huffman, PhD
Operations Manager: Dr. Kai Mao and Mr. Hongqian Liang
Consultants:
- Dr. Nir Barzilai (glucose metabolism)
- Dr. Gary Schwartz (Whole-animal metabolic phenotyping)
- Dr. Rajat Singh (Cell metabolism assays)
- Dr. Maria Gulinello (Rodent behavior phenotyping)
- Dr. Richard Kitsis (Cardiovascular phenotyping)
- Dr. Amanda Beck (Geropathology)
Services
Assays
- In vivo clamp studies
- Specialized surgeries (catheters, ICV cannulas, parabiosis)
- Metabolic phenotyping
- Behavioral and functional phenotyping
- Geropathology
- Hormonal assay
Reagents and Samples
- Aging and parabiotic tissue repository
Prices
| Type of Service | Member Rate | Non-Member Rate |
|---|---|---|
| qMR (rats or mice) | $10 | $30 |
| Micro CT scan | $50 | $100 |
| Complete dissection (weighing, fixation, freezing) | $20 | $35 |
| Type of Service | Member Rate | Non-Member Rate |
|---|---|---|
| Lipectomy | $100 | $150 |
| Clamp surgery | $100 | $150 |
| Inserting ICV or intra hypothalamic catheter | $100 | $150 |
| Chronobiosis Surgery+Housing Y-Y, Y-O, or O-O each | $200 | $300 |
| Chronobiosis Surgery+Housing (one set, i.e. Y-Y, Y-O, O-O) | $500 | $750 |
| Chronobiosis limited tissue request (one set, i.e., Y-Y, Y-O, O-Y, O-O) | $50 | $100 |
| Chronobiosis plasma/serum (one set, i.e., Y, Y-O, O-Y, O) ~50uL | $100 | $150 |
| Chronobiosis complete tissue and plasma (one set, i.e., Y-Y, Y-O, O-Y, O-O) | $600 | $900 |
| Type of Service | Member Rate | Non-Member Rate |
|---|---|---|
| Clamp assay: with radioisotope (mice) | $200 | $300 |
| Clamp assay: with radioisotope/C14 (mice) | $250 | $350 |
| Clamp assay: with radioisotope/C14 and ICV (mice) | $300 | $450 |
| Clamp assay: with radioisotope (rats) | $250 | $400 |
| Clamp assay: with radioisotope/C14 (rats) | $300 | $450 |
| Clamp assay: with radioisotope/C14 and ICV (rats) | $350 | $500 |
| Clamp data analysis | $15 | $25 |
| Mouse Metabolic Cages (8 chambers per week) | $75 | $125 |
| Rat Metabolic Cages (4 chambers per week) | $100 | $150 |
| Hormonal assays (kit and reagent costs apply) | inquire |
| Type of Service | Member Rate | Non-Member Rate |
|---|---|---|
| FUNCTIONAL ASSAYS | ||
| Balance Beam | $60/hr | $90/hr |
| Gait | $60/hr | $90/hr |
| Forced Exercise Test | $60/hr | $90/hr |
| Grip Strength | $60/hr | $90/hr |
| Rotarod | $60/hr | $90/hr |
| Open Field | $60/hr | $90/hr |
| Object Recognition | $70/hr | $120/hr |
| Object Placement | $70/hr | $120/hr |
| Y Maze/Zero Maze | $60/hr | $90/hr |
| Frailty assessment | $60/hr | $90/hr |
| Echocardiogram | $80/hr | $140/hr |
| Unassisted Core Room Use | $30/hr | inquire |
| Type of Service | Member Rate | Non-Member Rate |
|---|---|---|
| Geropathology analysis and report (4 tissues) | $51 | $100 |
| Tissue processing, embedding, sectioning and Geropathology (4 tissues) | $62.50 | $115 |
| Type of Service | Member Rate | Non-Member Rate |
|---|---|---|
| Consultation on study design, methods, interpretation | $75/hr | $120/hr |
| Training in Core methodologies | $60/hr | $100/hr |