The 3D Histech P250 High Capacity Slide Scanner is an automated high speed, high capacity slide scanner (up to 250 slides per batch), it has brightfield and fluorescence.
Attention: The NIH requires that all publications using data from this instrument must list SIG #1S10OD026852-01A1
Objectives:
20X NA=0.8 AIR
40X NA=0.95 AIR
Fluorescence Filters: Dapi, GFP, RFP, CY5
Image detection:
Brightfield (immunohistochemistry):
high-speed, 4 megapixel, color CMOS camera (VCC-FC60FR19CL, CIS Corp.)
Fluorescence
low-noise high-speed 5.5 megapixel CCD camera (PCO.edge, PCO Inc.)
Light Source:
Brightfield (immunohistochemistry):
stroboscopic illumination with slide traveling at constant velocity in xy (Lumencor Spectra)
Fluorescence
multi-color LED light source and quad band dichroic filter
Software: Pannoramic Scanner for acquisition. Case Viewer for visualization. Quant Center for analysis.
Additional Features: Z-stacking; Batch acquisition (250 Slides)
Special Rules for slides:
• Use hard mounting media only
• No nail polish (or other sealing agent), and No wet mount
• No cover slip protruding over slide edge
• No protruding labels
• Slides needs to be clean and dry: clean the back of the slide with ethanol and scrape the edges of the slide with a razor blade
• Use slide checker to make sure slide fits all parameters
• When circling sample with sharpie do so on the Cover Slip side
Instructions to download viewer software:
Case Viewer:
1. Go to: https://www.3dhistech.com/research/software/software-downloads/
2. Scroll to the middle of the page and click “Download CaseViewer 2.4 for Windows”
Note: This software does not work on Mac or Lynx computers.
Scale Bar Information
Gallery
Price 210