Analytical Imaging Facility

Hamamatsu Slide Scanner

The Hamamatsu Slide Scanner is an automated slide scanner. It provides brightfield and fluorescence scanning of whole slides.

Objectives:
20X NA=0.8 AIR

Fluorescence Filters: DAPI, 488, 555, mCherry, CY5, CY7

Light Source:
Brightfield (immunohistochemistry): Lumencor LED light source
Fluorescence: Lumencor LED light source

Image detection:
Brightfield (immunohistochemistry): high-speed 4-megapixel color CMOS camera
Fluorescence: low-noise high-speed 5.5-megapixel CCD camera

Software: NZ acquire for acquisition. NDP.View2 for visualization.

Additional Features: Z-stacking; Batch acquisition (60 slides)

Special Rules for Slides:

  • slides must be very clean
  • no overhanging coverglass
  • no overhanging stickers
  • coverglass must be attached (must not move easily):
    • hard-set mounting media
    • semi-wet/removable gelatin mount
    • no wet mount

Instructions to Download Viewer Software:

Click Here: https://www.hamamatsu.com/jp/en/product/life-science-and-medical-systems/digital-slide-scanner/U12388-01.html Scroll down to “Software and Manual Download”

Gallery
Forchheimer 655N