Human Islet and Adenovirus Core (located at Mt. Sinai)

^aCost is for culturing islets in one 6-well plate for one preparation. If more plates are required the discount will apply.

^bCost is for dispersing islets in one 24-well plate for one preparation. If more plates are required the discount will apply.

^cPrice for a single service, i.e. one in vitro cell proliferation, number, survival, transfection efficiency, GSIS >or mitochondrial function analysis experiment with three experimental conditions tested. There is a 20% discount per assay. If additional assays are requested, for example five GSIS experiments the charge is $400/$1300.

^dHuman islet transplant in one immunodeficient mouse. For additional transplanted mice there is a 20% discount per additional mouse. Weekly blood glucose measurements are included in the price. If mice need to be treated, the following cost applies:

• Tail vein injection: $8/mouse
• Intraperitoneal (ip) injection: $6/mouse
• Subcutaneous (sc) injection: $6/mouse
• Osmotic pump implant: $45/mouse
• Additional personnel time: $25/hr

^eHuman islet transplant assessment assays are:

• Islet graft harvest, fixation, embedding, sectioning and staining for insulin in three sections and beta cell area analysis: $100
• Islet graft harvest, fixation, embedding, sectioning and analysis of beta cell proliferation/death: $200
• IPGTT/OGTT, intraperitoneal or oral glucose tolerance test: $75 performed at MSSM
• hyperglycemic clamp and plasma insulin levels see APC and BARC Fee Schedule.

^fRodent islet isolation will be performed in mice ($70/mouse) or rats ($100/rat) using histopaque gradient. If islet handpicking is requested after purification, a $10 fee per animal will be charged. Any additional mouse or rat islet isolation will have the 20% discount.

^gCost of human beta cell sorting is per preparation and for two treatments, control and experimental. If no treatments are requested, cost will be half.