Kennedy Center Cellular and Molecular Shared Equipment Facility
The Rose Kennedy Building houses a Molecular Biology Core, a Surgical Suite and an Imaging Core. These cores, available to the faculty of the Department of Neuroscience, are found on the third, fifth, sixth and ninth floors; additional services are found throughout the building.
Molecular Biology Core (Room 328B&C)
This core includes instrumentation for routine molecular biology and biochemical procedures. It allows for sample preparation, genotyping and genomic profiling and documentation of results. [Access to these instruments will be charged $80 per person per day.]
Sample preparation
Centrifuges (rotors stored in 4°C refrigerator (2018))
- Ultracentrifuges
- Beckman L8-80M (rotors: SW-28 (28k rpm); type 35 (35k rpm); SW-40 Ti (40k rpm); 60 Ti (60k rpm); 70.2 Ti (70k rpm);)
- 3X high-speed centrifuges (rotors: JA-10 (10k rpm), KA-18.1, 70.2 Ti (70k rpm); SS-34)
- Beckman-Coulter Avanti J-E (2019)
- Beckman J2-21M
- Sorvall RC 5B Plus
- Eppendorf centrifuge 5430 R (14k rpm)
- LabConCo CentriVap DNA concentrator 7970000
Incubator shaker
- New Brunswick Excella E24
- New Brunswick Series 25
Tissue grinder/sonicator
- Misonix sonicator S-4000 with sound-proof enclosure (2018)
- Arrow Engineering tissue grinder
Boekel CCC 0.5d incubator
Vibratome Series 1000
Cryostats
- Leica CM3050S
- (2) Cryostar NX70 (2018, 2020)
Surgical Suite (Kennedy Room 328A&B)
3x Stereotaxic rigs (adult, juvenile, pathogen) (new):
- Olympus SZX7 microscope
- RWD stereotaxic positioner
- Drummond Nanoject III
- RWD anesthesia machine (Isoflurane included)
- Thermostar warmer
Biosafety cabinets (Class IIA/B3)
- Nuaire NU-245FM-400
- Forma Scientific 1184
- Baker Company 2340A-ATS
Fume hood
Sutter P-87 Flaming/Brown micropipette puller
Sutter P-2000 laser micropipette puller (2020 rebuilt)
Vibratome
Electrophysiology rig (Room 329A)
- Olympus Fluoview 1000 laser scanning confocal microscope
Genotyping & genomic profiling
PCR
- Applied Biosystems GeneAmp System 9700 (0.2ml reaction tubes or 96 & 384 well reaction plates)
- BioRad CFX96 real-time PCR (2018)
- Applied Biosystems 7300 Real-Time PCR
Documentation of results
Gel/Blot scan
- Bio-Rad Gel Doc XR+
- Licor Odyssey CLx
- Azure c600 (2018)
Plate reader
- Tecan Infinite M200 pro (2018)
Scintillation counter
Additional services
[Except where stated, access to these instruments will be charged $80 per person per day.]
Autoclave – Room 423, 614
Dishwasher – Rooms 423, 614
Liquid Nitrogen – Room 614
Milli-Q® IQ 7000 Ultrapure Water System (2019) –Room 328C
70% Ethanol - Room 328C
Ice Machine – Room 450 [free access]
Emergency storage – Room 328B
- freezer (-20°C) [$100 per month per shelf or partial shelf]
Emergency Storage – basement (Room B51)
- 3x freezers (-80°C) [$200 per month per shelf or partial shelf]
