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Stem Cell Isolation, Xenotransplantation and PDX Unit
Our unit provides specialized services and advanced research support for scientists working in stem cell biology, cancer research, regenerative medicine, and translational therapeutics.
Our unit enables isolation, enrichment, characterization, and culture of stem cells from diverse biological samples, supporting both basic and applied biomedical research. The facility also offers expertise in xenotransplantation models, allowing researchers to study human cells and tissues in vivo for applications such as disease modeling, stem cell engraftment, tumor biology, drug response, and therapeutic evaluation. In addition, our unit develops and maintains Patient-Derived Xenograft (PDX) models, which preserve molecular and clinical characteristics of primary tissues and cells, providing highly relevant platforms for precision medicine, biomarker discovery, and preclinical drug testing.
Through state-of-the-art infrastructure, technical expertise, and collaborative support, the core accelerates the translation of laboratory discoveries into clinically relevant healthcare innovations.
Instruments
- Five laser Cytek Aurora CS full spectrum high speed cell sorter with capacity of >40 color panel and 6 way, 96 well place sorting (located in Chanin 638A and Price 118)
- Five laser 15 color BD Aria II high speed cell sorter (located in Price 118)
Flow Cytometry Services
- Fluorescence-Activated Cell Sorting (FACS) of human and mouse stem and progenitor cells, and from various types of developmental sources and tissue origins.
- Sorting of cancer stem/ initiating cells and other human cell types associated with tumor microenvironment (i.e. macrophages, breast myo-epithelial cells, T / B cells, etc.) obtained from solid tumors (i.e. breast, ovary, endometrium, liver, bone marrow).
Xenotransplantation Services
- Xenograft Studies: Establish and propagate xenograft models from cell lines and patient samples using multiple implantation routes for downstream research.
- Efficacy Studies: Evaluate therapeutic response of agents in murine tumors, human xenografts, and transgenic cancer models.
- Compound Delivery: Administer test compounds in vivo via oral, intravenous, intraperitoneal, and dietary routes.
- Rodent Toxicology: Determine maximum tolerated dose and assess safety through single and repeat dose studies.
- Sample Collection: Collect blood, live bone marrow aspirations for analysis of engraftment and treatment response.
PDX (Patient-Derived Xenograft) Services
- Generation of Patient Derived Xenograft (PDX) models
- Implantation of patient tumor tissues into immunodeficient mice
- Expansion and maintenance of PDX colonies
- Cryopreservation and biobanking of PDX samples
- Preclinical drug screening and therapeutic testing
- Tumor passage and monitoring
- PDX derived samples distribution for collaborative research
- Consultation of Stem Cell Isolation, Xenotransplantation and PDX protocols.