Head of Eukaryotic Protein Production in the Macromolecular Therapeutics Development Facility. Our goal is the production of many different proteins, primarily for structural analysis, from our eukaryotic platforms.

Garforth S.J., Domaoal R.A.*, Lwatula C., Landau M.J., Meyer A.J., Anderson K.S., and Prasad V.R. (2010) K65R and K65A substitutions in HIV-1 reverse transcriptase enhance polymerase fidelity by decreasing both dNTP misinsertion and mispaired primer extension efficiencies. J. Mol. Biol. 401 33-44. (* joint first author)

Baughn A.D., Garforth S.J., Vilchèze C., and Jacobs W.R. (2009) An anaerobic-type a-ketoglutarate: ferredoxin oxidoreductase completes the oxidative tricarboxylic acid cycle of Mycobacterium tuberculosis. PloS Pathog. 5 e1000662. 

Garforth S.J., Parniak M.A. and Prasad V.R. (2008) Utilization of a deoxynucleoside diphosphate substrate by HIV-1 reverse transcriptase. PLoS ONE. 3 e2074.

Silverman A.P., Garforth S.J., Prasad V.R., Kool E.T. (2008) Probing the active site steric flexibility of HIV-1 reverse transcriptase: Different constraints for DNA- versus RNA-templated synthesis. Biochemistry. 47 4800-7.

Garforth S.J., Kim T.W., Parniak M.A., Kool E.T. and Prasad V.R. (2007) Site-directed mutagenesis in the fingers subdomain of HIV-1 reverse transcriptase reveals a specific role for the beta3-beta4 hairpin loop in dNTP selection. J. Mol. Biol. 365 38-49.

Glover R.T., Kriakov J., Garforth S.J., Baughn A.D. and Jacobs W.R. (2007) The two-component regulatory system senX3-regX3 regulates phosphate-dependent gene expression in Mycobacteria smegmatis. J. Bacteriol. 189 5495-5503. 

Garforth S.J., Wu Y.Y. and Prasad V.R. (2006) Structural features of mouse telomerase RNA are responsible for the lower activity of mouse telomerase versus human telomerase. Biochem. J. 397 399-406.