shRNA Resources

A summary of reagent sources for lentiviral packaging, and information on protocols and controls, prepared by the shRNA Core Facility

December 2016: Summary of CRISPR services.

March 2015: Expansion of CRISPR services. 

October 2014: Introduction of new services for generation of custom cell lines stably knocked out for particular genes.

May 2014: An overview of the capabilities of the Operetta instrument, and changes to pricing for some services.

April 2014: Summary of arrayed and pooled screens available for gene modulation using shRNA, CRISPR/Cas9, and ORF approaches.

John Reidhaar-Olson, Ph.D., Operations Director, shRNA Core Facility

Deborah Smith, Ph.D., shRNA Core Facility
February 29, 2012

Paul Fletcher, Ph.D., Field Applications Scientist, Life Sciences & Technology, PerkinElmer Inc.
March 28, 2012

Supplier of shRNA and ORF reagents and libraries, including the Einstein GIPZ, TRC and LentiORF libraries.

Information about The RNAi Consortium shRNA collection.

Information about the sequence-validated human ORF clone that comprise the LentiORF library.

Portal for shRNA reagents from the TRC collection. Notes which have been validated.

Database of phenotypes from RNAi screens in human and Drosophila cells, maintained by the German Cancer Research Center (DKFZ) of Heidelberg University.