Recursos de shRNA
shRNA Core Facility Newsletters
Cell line Gene Modulation Services from the shRNA/CRISPR Core Facility
December 2016: Summary of CRISPR services.
New CRISPR Cell Line Services: Knockout, Knockin, Activation, Repression
March 2015: Expansion of CRISPR services.
New CRISPR Services in the shRNA Core Facility
October 2014: Introduction of new services for generation of custom cell lines stably knocked out for particular genes.
shRNA Core Facility News: High-content screening
May 2014: An overview of the capabilities of the Operetta instrument, and changes to pricing for some services.
Gene function screens in the shRNA Core Facility
April 2014: Summary of arrayed and pooled screens available for gene modulation using shRNA, CRISPR/Cas9, and ORF approaches.
Past RNAi User Group and Other Presentations
An introduction to the shRNA Core Facility
John Reidhaar-Olson, Ph.D., Operations Director, shRNA Core Facility
shRNA: The Basics. A walk-through of steps and decision points for planning an shRNA experiment and ordering the appropriate reagents
Deborah Smith, Ph.D., shRNA Core Facility
February 29, 2012
Cellular and Biochemical Assays Utilizing PerkinElmer Technologies: Applications for RNAi Research
Paul Fletcher, Ph.D., Field Applications Scientist, Life Sciences & Technology, PerkinElmer Inc.
March 28, 2012
External Resources
Open Biosystems
Supplier of shRNA and ORF reagents and libraries, including the Einstein GIPZ, TRC and LentiORF libraries.
Public TRC Portal
Information about The RNAi Consortium shRNA collection.
The ORFeome Collaboration
Information about the sequence-validated human ORF clone that comprise the LentiORF library.
Sigma gene search
Portal for shRNA reagents from the TRC collection. Notes which have been validated.
GenomeRNAi
Database of phenotypes from RNAi screens in human and Drosophila cells, maintained by the German Cancer Research Center (DKFZ) of Heidelberg University.