shRNA Resources
shRNA Core Facility Newsletters
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Cell line Gene Modulation Services from the shRNA/CRISPR Core Facility
December 2016: Summary of CRISPR services.
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New CRISPR Cell Line Services: Knockout, Knockin, Activation, Repression
March 2015: Expansion of CRISPR services.
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New CRISPR Services in the shRNA Core Facility
October 2014: Introduction of new services for generation of custom cell lines stably knocked out for particular genes.
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shRNA Core Facility News: High-content screening
May 2014: An overview of the capabilities of the Operetta instrument, and changes to pricing for some services.
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Gene function screens in the shRNA Core Facility
April 2014: Summary of arrayed and pooled screens available for gene modulation using shRNA, CRISPR/Cas9, and ORF approaches.
Past RNAi User Group and Other Presentations
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An introduction to the shRNA Core Facility
John Reidhaar-Olson, Ph.D., Operations Director, shRNA Core Facility
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shRNA: The Basics. A walk-through of steps and decision points for planning an shRNA experiment and ordering the appropriate reagents
Deborah Smith, Ph.D., shRNA Core Facility
February 29, 2012 -
Cellular and Biochemical Assays Utilizing PerkinElmer Technologies: Applications for RNAi Research
Paul Fletcher, Ph.D., Field Applications Scientist, Life Sciences & Technology, PerkinElmer Inc.
March 28, 2012
External Resources
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Open Biosystems
Supplier of shRNA and ORF reagents and libraries, including the Einstein GIPZ, TRC and LentiORF libraries.
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Public TRC Portal
Information about The RNAi Consortium shRNA collection.
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The ORFeome Collaboration
Information about the sequence-validated human ORF clone that comprise the LentiORF library.
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Sigma gene search
Portal for shRNA reagents from the TRC collection. Notes which have been validated.
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GenomeRNAi
Database of phenotypes from RNAi screens in human and Drosophila cells, maintained by the German Cancer Research Center (DKFZ) of Heidelberg University.