shRNA Resources Protocols and Other Information Reagents and Information A summary of reagent sources for lentiviral packaging, and information on protocols and controls, prepared by the shRNA Core Facility shRNA Core Facility Newsletters Cell line Gene Modulation Services from the shRNA/CRISPR Core FacilityDecember 2016: Summary of CRISPR services. New CRISPR Cell Line Services: Knockout, Knockin, Activation, RepressionMarch 2015: Expansion of CRISPR services. New CRISPR Services in the shRNA Core FacilityOctober 2014: Introduction of new services for generation of custom cell lines stably knocked out for particular genes. shRNA Core Facility News: High-content screeningMay 2014: An overview of the capabilities of the Operetta instrument, and changes to pricing for some services. Gene function screens in the shRNA Core FacilityApril 2014: Summary of arrayed and pooled screens available for gene modulation using shRNA, CRISPR/Cas9, and ORF approaches. Past RNAi User Group and Other Presentations An introduction to the shRNA Core Facility John Reidhaar-Olson, Ph.D., Operations Director, shRNA Core Facility January 25, 2012 shRNA: The Basics. A walk-through of steps and decision points for planning an shRNA experiment and ordering the appropriate reagents Deborah Smith, Ph.D., shRNA Core Facility February 29, 2012 Cellular and Biochemical Assays Utilizing PerkinElmer Technologies: Applications for RNAi Research Paul Fletcher, Ph.D., Field Applications Scientist, Life Sciences & Technology, PerkinElmer Inc. March 28, 2012 External Resources Open Biosystems Supplier of shRNA and ORF reagents and libraries, including the Einstein GIPZ, TRC and LentiORF libraries. Public TRC Portal Information about The RNAi Consortium shRNA collection. The ORFeome Collaboration Information about the sequence-validated human ORF clone that comprise the LentiORF library. Sigma gene search Portal for shRNA reagents from the TRC collection. Notes which have been validated. GenomeRNAi Database of phenotypes from RNAi screens in human and Drosophila cells, maintained by the German Cancer Research Center (DKFZ) of Heidelberg University.
