Robert H. Singer, Ph.D.
- Professor, Department of Cell Biology
- Professor, Dominick P. Purpura Department of Neuroscience
- Harold and Muriel Block Chair in Anatomy & Structural Biology
Area of research
- We study single mRNA molecules from cradle to grave in single living cells using high resolution imaging. We develop trafficking microscopy and approaches to image RNA using fluorescent probes to visualize and quantify mRNA.
Phone
Location
- Albert Einstein College of Medicine Jack and Pearl Resnick Campus 1300 Morris Park Avenue Golding Building 601 Bronx, NY 10461
Research Profiles
Professional Interests
Studying Single mRNA Molecules from Cradle to Grave
We seek to understand the expression and movement of mRNA from transcription through degradation and the effect that defects in these processes have on health. We develop methods to label RNA in fixed and living cells using fluorescent probes and develop microscopy techniques and image analysis algorithms to visualize and quantify many mRNAs simultaneously. Using these technologies, we can observe single mRNAs localizing to cytoplasmic compartments such as the leading edge of a fibroblast, the bud tip of yeast or the axonal process of neurons and follow these single mRNAs from transcription, nuclear export through translation and degradation. Because these techniques yield quantitative fluorescence data, we are able to apply mathematical modeling to test mechanistic hypotheses.
RNA Localization: Following Single mRNAs from Birth to Death in Living Cells