Our laboratory specializes in the use of fluorescence imaging techniques to answer questions fundamental to liver biology. We have developed in vitro organelle motility assays to study the molecular mechanisms of intracellular protein sorting and viral trafficking that occurs along the microtubule cytoskeleton. I have also developed single cell assays for monitoring the uptake, accumulation, and secretion of bile acids, and we are studying how drugs, hormones, and cellular signaling may specifically regulate these steps. We hypothesize that the trafficking of bile acid transporters to and from the cell surface can affect bile acid homeostasis, potentially affecting uptake and metabolism of drugs. Additionally we are interested in screening and developing fluorescent labeled probes that can be used to characterize the 'transport state' of cells, which can also reflect the susceptibility of cells to drugs.

Peterson EA, Polgar Z, Devakanmalai GS, Li Y, Jaber FL, Zhang W, Wang X, Iqbal NJ, Murray JW, Roy-Chowdhury N, Quispe-Tintaya W, Maslov AY, Tchaikovskaya TL, Sharma Y, Rogler LE, Gupta S, Zhu L, Roy-Chowdhury J, Shafritz DA. Genes and Pathways Promoting Long-Term Liver Repopulation by Ex Vivo hYAP-ERT2 Transduced Hepatocytes and Treatment of Jaundice in Gunn Rats. Hepatol Commun. 2019 Jan;3(1):129-146. doi: 10.1002/hep4.1278. eCollection 2019 Jan. PubMed PMID: 30620000; PubMed Central PMCID: PMC6312667.

Bejarano E, Murray JW, Wang X, Pampliega O, Yin D, Patel B, Yuste A, Wolkoff AW, Cuervo AM. Defective recruitment of motor proteins to autophagic compartments contributes to autophagic failure in aging. Aging Cell. 2018 Aug;17(4):e12777. doi: 10.1111/acel.12777. Epub 2018 May 29. PubMed PMID: 29845728; PubMed Central PMCID: PMC6052466.

Murray JW, Yin D, Wolkoff AW. 2017. Reduction of organelle motility by removal of potassium and other solutes. PLoS One. Sep 18;12(9):e0184898.

Wang X, Wang P, Wang W, Murray JW, Wolkoff AW. 2016. The Na(+)-Taurocholate Cotransporting Polypeptide Traffics with the Epidermal Growth Factor Receptor. Traffic. Mar;17(3):230-44.

Murray JW, Han D, Wolkoff AW. 2014. Hepatocytes maintain greater fluorescent bile acid accumulation and greater sensitivity to drug-induced cell death in three-dimensional matrix culture. Physiol Rep. Dec 18;2(12).

Murray JW, Thosani AJ, Wang P, Wolkoff AW. 2011. Heterogeneous accumulation of fluorescent bile acids in primary rat hepatocytes does not correlate with their homogenous expression of ntcp. Am J Physiol Gastrointest Liver Physiol. 2011 Jul;301(1):G60-8.

Fort AG, Murray JW, Dandachi N, Davidson MW, Dermietzel R,Wolkoff AW, Spray DC. 2011. In vitro motility of liver connexin vesicles along microtubules utilizes kinesin motors. J Biol Chem. Jul 1;286(26):22875-85

 MurrayJW, Thosani AJ, Wang P, Wolkoff AW. 2011. Heterogeneous accumulation of fluorescent bile acids in primary rat hepatocytes does not correlate with their homogenous expression of ntcp.  Am J Physiol Gastrointest Liver Physiol. Jul;301(1):G60-8

 Mukhopadhyay A, Nieves E, Che FY, Wang J, Jin L, Murray JW, Gordon K, Angeletti RH, Wolkoff AW. 2011. Proteomic analysis of endocytic vesicles: Rab1a regulates motility of early endocytic vesicles. J Cell Sci. Mar 1;124(Pt 5):765-75.

Murray JW, Wolkoff AW. 2007. In vitro motility system to study the role of motor proteins in receptor-ligand sorting. Methods Mol Biol;392:143-58.

 Sarkar S, Bananis E, Nath S, Anwer MS, Wolkoff AW, Murray, JW. 2006. PKCzeta is required for microtubule-based motility of vesicles containing the ntcp transporter. Traffic. Aug;7(8):1078-91.

 Lee GE, Murray JW, Wolkoff AW, Wilson DW. 2006. Reconstitution of herpes simplex virus microtubule-dependent trafficking in vitro. J. Virol. 80:4264-75.

 Bananis, E, Murray, JW, Stockert RJ, Satir P, Wolkoff AW. 2000. Microtubule and motor-dependent endocytic vesicle sorting in vitro. J. Cell Biol. 151 (1):179-186.

Murray JW, Edmonds BT, Liu G, Condeelis J. 1996. Bundling of actin filaments by elongation factor 1 alpha inhibits polymerization at filament ends. J. Cell Biol. 135:1309-1321.

 Murray J, Vawter-Hugart H, Voss E, Soll DR. 1992. Three-dimensional motility cycle in leukocytes. Cell Motil. Cytoskel. 22:211-23.