Research project summary:

Our lab have focused its interest on autophagy and T cell function for the last decade. My initial studies in that field focused more specifically on the regulation of macroautophagy and T cell function. We have notably found, that following T cell activation, common-gamma chain receptor cytokines are require to induce and maintain hight autophagy rate which is require for proper T cell function.

currently we are investigating the consequences of autophagy-deficient T cell in non-lymphoid organs in mice models.

We are also exploring the discovery of new autophagy regulators.

12- Michael A Willcockson, Sean E Healton, Cary N Weiss, Boris A Bartholdy, Yair Botbol, Laxmi N Mishra, Dhruv S Sidhwani, Tommy J Wilson, Hugo B Pinto, Maxim I Maron, Karin A Skalina, Laura Norwood Toro, Jie Zhao, Chul-Hwan Lee, Harry Hou, Nevin Yusufova, Cem Meydan, Adewola Osunsade, Yael David, Ethel Cesarman, Ari M Melnick, Simone Sidoli, Benjamin A Garcia, Winfried Edelmann, Fernando Macian, Arthur I Skoultchi, “H1 histones control the epigenetic landscape by local chromatin compaction”, Nature, December 2020. 5-Yr IF 46.486

11-Enric Mocholi, Samuel D.Dowling, Yair Botbol, Ross C.Gruber, Alex K.Ray SebastiaanVastert, Bridget Shafit-Zagardo, Paul J.Coffer, FernandoMacian. “Autophagy Is a Tolerance-Avoidance Mechanism that Modulates TCR-Mediated Signaling and Cell Metabolism to Prevent Induction of T Cell Anergy”, Cell Report, 24(5):1136-1150, July 2018, 5-yrs IF 8.70

10- Genevieve Marcelin, Adaliene Ferreirra, Yuejun Liu, Michael Atlan, Judith Aron-

Wisnewsky, Veronique Pelloux, Yair Botbol, Marc Ambrosini, Magali Fradet, Christine Rouault, Corneliu Hénégar, Jean-Sebastien Hulot, Christine Poitou, Adriana Torcivia, Raphael Nail-Barthelemy, Jean-Christophe Bichet, Emmanuel L. Gautier, Karine Clement. "PDGFRα-mediated switch to CD9high progenitors controls fibrotic adipose tissue alteration in obesity” Cell Metabolism, 25(3):673-685, February 2017, 5-Yr IF 17.897

9- Yair Botbol, Guerrero-Ros I & Fernando Macian,

"Key roles of autophagy in regulating T-cell function", European  Journal of Immunology review, 46(6):1326-34, May 2016, 5-Yr IF 4.278

8- AnnMarie Torres , Joanna D Luke , Amy L Kullas , Kanishk Kapilashrami , Yair Botbol , Antonius Koller , Peter J Tonge , Emily I. Chen , Fernando Macian , Adrianus WM van der Velden

"L-asparagine deprivation mediated by Salmonella L-Asparaginase causes suppression of activation-induced T cell metabolic reprogramming" Journal of Leukocyte Biology , 99(2):387-98, February 2016, 5-Yr IF 4.705

7- Yair Botbol, Bindi Patel & Fernando Macian,

"Common γ-chain cytokine signaling is required for macroautophagy induction during CD4+ T cell activation", Autophagy, 11(10):1864-77, September 2015, 5-Yr IF 10.698

6- Yair Botbol & Fernando Macian

"Assays for monitoring macroautophagy activity in T cells", Methods in Molecular Biology, 1343:143-53, September 2015, IF 1.29

5- Rut Valdor, Enric Mocholi, Yair Botbol, Ignacio Guerrero-Ros, Dinesh Chandra, Hiroshi Koga, Claudia Gravekamp, Ana Maria Cuervo         & Fernando Macian.

"Chaperone mediated autophagy regulates T cell responses through targeted degradation of negative regulators of T cell activation", Nature Immunology, 15(11):1046-54, November 2014, 5-Yr IF 23.956

4- Sridhar S, Botbol Y, Macian F, Cuervo AM.

Autophagy and disease: always two sides to a problem. Journal of Pathology. review, 226(2):255-73, January 2012, 5-Yr IF 6.94

3- Paul Lesbats, Yair Botbol, Guillaume Cheverau, Cédric Vaillant, Christina Calmels, Alain Arnéodo, Marie-Line Andreola, Marc Lavigne and Vincent Parissi

“Functional coupling between HIV-1 integrase and the SWI/SNF chromatin remodeling complex for efficient in vitro integration into stable nucleosomes” Plos. Pathogen, 10;7(2):e1001280, February 2011, IF 8.057

2- Ming-Chieh Shun, Yaïr Botbol, Xiang Li, Francesca Di Nunzio, Janet E. Daigle, Nan Yan, Judy Lieberman, Marc Lavigne, and Alan Engelman.

“Identification and Characterization of PWWP Domain Residues Critical for LEDGF/p75 Chromatin-Binding and Human Immunodeficiency Virus Type 1 Infectivity” Journal of Virology, 82(23):11555-67, December 2008, 5-Yr IF 4.609

1- Botbol Y, Raghavendra NK, Rahman S, Engelman A, Lavigne M.

“Chromatinized templates reveal the requirement for the LEDGF/p75 PWWP domain during HIV-1 integration in vitro”. Nucleic Acid Research, 36(4):1237-46, Mars 2008, 5-Yr IF 8.867