Hybridoma

The Hybridoma Facility produces mouse monoclonal antibodies to antigens provided by investigators. For most investigators, the staff of the facility carries out the entire process from mouse immunization through fusion for hybridoma generation, cloning, screening, production of monoclonal antibody (mAb), and freezing of cell lines for long-term storage. In general, facility staff meet with the Principal Investigator, or his or her representative, to discuss the scientific goals of the project and to make decisions regarding immunogen preparation, immunization strategy, screening for positive hybridomas once a fusion is completed, and the types of antibodies required. Investigators provide the antigen for immunization and screening, while the facility provides and maintains the mice and performs all steps from immunization through fusion, cloning, and primary screening.

In cases where screening for positive hybridomas requires specialized expertise, members of the PI’s laboratory may participate in or coordinate with the facility on the screening process. Subsequent cloning and freezing are performed by the facility, and key clones are stored as a backup for investigators. During the course of hybridoma production, students and fellows may observe the process to gain experience with hybridoma technology. The facility also provides advice and letters of support for grant applications involving the production and use of monoclonal antibodies.