David R. Entenberg, Ph.D.

David Entenberg’s research interests are in the development of enabling technologies for biological research. With a background in laser based experimental quantum physics he brings skills in optical, mechanical, electrical, software and instrument design to biological research. Previous work has included the design and development of several robotics-based high-throughput automated biological assays utilizing real time PCR and MALDI-TOF protocols, novel microscopes including a fast switching, multi-channel TIRF microscope, a video rate multiphoton microscope, and a two-laser, OPO based multiphoton microscope. Current projects are focused on utilizing surgical engineering to develop and validate techniques for expanding the utility of mouse models within intravital imaging. This work has included mammary, abdominal, brain, lymphatic and lung imaging windows for studying cancer metastasis to secondary sites in vivo, as well surgically implantable photolithographic microdevices designed to directly alter the local (<300um) microenvironment in vivo and during intravital imaging.

He has authored several book chapters on multiphoton microscopy and intravital imaging and is co-Director of Einstein’s Quantitative Imaging of Cells course. He currently serves as co-Director of Einstein's Gruss Lipper Biophotonics Center and Leader of the Computational Analysis of Biomarkers Program within the Integrated Imaging Program for Cancer Research.

My Bibliography

1. Zambalde EP, Ariyan LA, Puerta-Martinez F, Zhu YQ, Patil P, Panarelli NC, Li J, Jung Y, Adkisson C, Petersen J, Cox D, Gil-Henn H, Eddy R, Stanger BZ, Entenberg D, Oktay MH, Condeelis JS, McAuliffe JC. Tie2 inhibition disrupts TMEM doorway function and reduces dissemination in pancreatic ductal adenocarcinoma. J Exp Clin Cancer Res. 2026. Epub 20260516. doi: 10.1186/s13046-026-03730-6. PubMed PMID: 42143388.
2. Parmar P, Karadal-Ferrena B, Shukla S, Miller A, Zhang C, Huang C, D'Alfonso T, Han R, Adler E, Ladak N, Ginter PS, Fineberg S, Ye X, Ginsberg M, Rosenbaum C, Felder M, Lin Y, Chen X, Eddy RJ, Rohan TE, Condeelis JS, Xue X, Anampa J, Sparano JA, Entenberg D, Oktay MH. Racial disparity in pro-metastatic tumor microenvironment in treatment naive breast cancer. NPJ Breast Cancer. 2026;12(1):3. Epub 20260106. doi: 10.1038/s41523-025-00865-1. PubMed PMID: 41495055; PMCID: PMC12780041.
3. Lucotti S, Ogitani Y, Kenific CM, Geri J, Kim YH, Gu J, Balaji U, Bojmar L, Shaashua L, Song Y, Cioffi M, Lauritzen P, Joseph OM, Asao T, Grandgenett PM, Hollingsworth MA, Peralta C, Pagano AE, Molina H, Lengel HB, Dunne EG, Jing X, Schmitter M, Borriello L, Miller T, Zhang H, Romin Y, Manova K, Paul D, Remmel HL, O'Reilly EM, Jarnagin WR, Kelsen D, Castellino SM, Giulino-Roth L, Jones DR, Condeelis JS, Pascual V, Bussel JB, Boudreau N, Matei I, Entenberg D, Bromberg JF, Simeone DM, Lyden D. Extracellular vesicles from the lung pro-thrombotic niche drive cancer-associated thrombosis and metastasis via integrin beta 2. Cell. 2025. Epub 20250210. doi: 10.1016/j.cell.2025.01.025. PubMed PMID: 39938515.
4. Duran CL, Surve CR, Ye X, Chen X, Lin Y, Harney AS, Wang Y, Sharma VP, Stanley ER, Cox D, McAuliffe JC, Entenberg D, Oktay MH, Condeelis JS. Targeting CSF-1 signaling between tumor cells and macrophages at TMEM doorways inhibits breast cancer dissemination. Oncogene. 2025;44(36):3297-309. Epub 20250711. doi: 10.1038/s41388-025-03485-y. PubMed PMID: 40646332; PMCID: PMC12399421.
5. Dalla E, Papanicolaou M, Park MD, Barth N, Hou R, Segura-Villalobos D, Valencia Salazar L, Sun D, Forrest ARR, Casanova-Acebes M, Entenberg D, Merad M, Aguirre-Ghiso JA. Lung-resident alveolar macrophages regulate the timing of breast cancer metastasis. Cell. 2024;187(23):6631-48 e20. Epub 20241007. doi: 10.1016/j.cell.2024.09.016. PubMed PMID: 39378878; PMCID: PMC11568918.
6. Chioccioli M, Liu S, Magruder S, Tata A, Borriello L, McDonough JE, Konkimalla A, Kim SH, Nouws J, Gonzalez DG, Traub B, Ye X, Yang T, Entenberg DR, Krishnaswamy S, Hendry CE, Kaminski N, Tata PR, Sauler M. Stem cell migration drives lung repair in living mice. Dev Cell. 2024. Epub 20240214. doi: 10.1016/j.devcel.2024.02.003. PubMed PMID: 38377991.
7. Kim G, Karadal-Ferrena B, Qin J, Sharma VP, Oktay IS, Lin Y, Ye X, Asiry S, Pastoriza JM, Cheng E, Ladak N, Condeelis JS, Adler E, Ginter PS, D'Alfonso T, Entenberg D, Xue X, Sparano JA, Oktay MH. Racial disparity in tumor microenvironment and distant recurrence in residual breast cancer after neoadjuvant chemotherapy. NPJ Breast Cancer. 2023;9(1):52. Epub 20230613. doi: 10.1038/s41523-023-00547-w. PubMed PMID: 37311792; PMCID: PMC10264351.
8. Entenberg D, Oktay MH, Condeelis JS. Intravital imaging to study cancer progression and metastasis. Nat Rev Cancer. 2023;23(1):25-42. Epub 20221116. doi: 10.1038/s41568-022-00527-5. PubMed PMID: 36385560; PMCID: PMC9912378.
9. Duran CL, Karagiannis GS, Chen X, Sharma VP, Entenberg D, Condeelis JS, Oktay MH. Cooperative NF-kappaB and Notch1 signaling promotes macrophage-mediated MenaINV expression in breast cancer. Breast Cancer Res. 2023;25(1):37. Epub 20230406. doi: 10.1186/s13058-023-01628-1. PubMed PMID: 37024946; PMCID: PMC10080980.
10. Selvanesan BC, Chandra D, Quispe-Tintaya W, Jahangir A, Patel A, Meena K, Alves Da Silva RA, Friedman M, Gabor L, Khouri O, Libutti SK, Yuan Z, Li J, Siddiqui S, Beck A, Tesfa L, Koba W, Chuy J, McAuliffe JC, Jafari R, Entenberg D, Wang Y, Condeelis J, DesMarais V, Balachandran V, Zhang X, Lin K, Gravekamp C. Listeria delivers tetanus toxoid protein to pancreatic tumors and induces cancer cell death in mice. Sci Transl Med. 2022;14(637):eabc1600. Epub 20220323. doi: 10.1126/scitranslmed.abc1600. PubMed PMID: 35320003; PMCID: PMC9031812.
11. Scheele C, Herrmann D, Yamashita E, Celso CL, Jenne CN, Oktay MH, Entenberg D, Friedl P, Weigert R, Meijboom FLB, Ishii M, Timpson P, van Rheenen J. Multiphoton intravital microscopy of rodents. Nat Rev Methods Primers. 2022;2(1):1-26. Epub 20221110. doi: 10.1038/s43586-022-00168-w. PubMed PMID: 37621948; PMCID: PMC10449057.
12. Karagiannis GS, Bianchi A, Sanchez LR, Ambadipudi K, Cui MH, Anampa JM, Asiry S, Wang Y, Harney AS, Pastoriza JM, Lin Y, Chen X, Jones JG, Entenberg D, Haddad D, Hodges LJ, Duong TQ, Sparano JA, Oktay MH, Branch CA, Condeelis JS. Assessment of MRI to estimate metastatic dissemination risk and prometastatic effects of chemotherapy. NPJ Breast Cancer. 2022;8(1):101. Epub 20220902. doi: 10.1038/s41523-022-00463-5. PubMed PMID: 36056005; PMCID: PMC9440218.
13. Borriello L, Coste A, Traub B, Sharma VP, Karagiannis GS, Lin Y, Wang Y, Ye X, Duran CL, Chen X, Friedman M, Sosa MS, Sun D, Dalla E, Singh DK, Oktay MH, Aguirre-Ghiso JA, Condeelis JS, Entenberg D. Primary tumor associated macrophages activate programs of invasion and dormancy in disseminating tumor cells. Nat Commun. 2022;13(1):626. Epub 2022/02/04. doi: 10.1038/s41467-022-28076-3. PubMed PMID: 35110548; PMCID: PMC8811052.
14. Sharma VP, Tang B, Wang Y, Duran CL, Karagiannis GS, Xue EA, Entenberg D, Borriello L, Coste A, Eddy RJ, Kim G, Ye X, Jones JG, Grunblatt E, Agi N, Roy S, Bandyopadhyaya G, Adler E, Surve CR, Esposito D, Goswami S, Segall JE, Guo W, Condeelis JS, Wakefield LM, Oktay MH. Live tumor imaging shows macrophage induction and TMEM-mediated enrichment of cancer stem cells during metastatic dissemination. Nat Commun. 2021;12(1):7300. Epub 2021/12/17. doi: 10.1038/s41467-021-27308-2. PubMed PMID: 34911937; PMCID: PMC8674234.
15. Du W, Adkisson C, Ye X, Duran CL, Chellakkan Selvanesan B, Gravekamp C, Oktay MH, McAuliffe JC, Condeelis JS, Panarelli NC, Norgard RJ, Sela Y, Stanger BZ, Entenberg D. SWIP-a stabilized window for intravital imaging of the murine pancreas. Open Biol J. 2022;12(6):210273. Epub 20220615. doi: 10.1098/rsob.210273. PubMed PMID: 35702996; PMCID: PMC9198798.
16. Entenberg D, Voiculescu S, Guo P, Borriello L, Wang Y, Karagiannis GS, Jones J, Baccay F, Oktay M, Condeelis J. A permanent window for the murine lung enables high-resolution imaging of cancer metastasis. Nat Methods. 2018;15(1):73-80. Epub 20171127. doi: 10.1038/nmeth.4511. PubMed PMID: 29176592; PMCID: PMC5755704.
17. Harper KL, Sosa MS, Entenberg D, Hosseini H, Cheung JF, Nobre R, Avivar-Valderas A, Nagi C, Girnius N, Davis RJ, Farias EF, Condeelis J, Klein CA, Aguirre-Ghiso JA. Mechanism of early dissemination and metastasis in Her2(+) mammary cancer. Nature. 2016;540:589-612. doi: 10.1038/nature20609. PubMed PMID: 27974798; PMCID: 5471138.
18. Lim SJ, Zahid MU, Le P, Ma L, Entenberg D, Harney AS, Condeelis J, Smith AM. Brightness-equalized quantum dots. Nat Commun. 2015;6:8210. doi: 10.1038/ncomms9210. PubMed PMID: 26437175; PMCID: 4594210.
19. Entenberg D, Wyckoff J, Gligorijevic B, Roussos ET, Verkhusha VV, Pollard JW, Condeelis J. Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging. Nat Protoc. 2011;6(10):1500-20. Epub 2011/10/01. doi: 10.1038/nprot.2011.376. PubMed PMID: 21959234; PMCID: 4028841.
20. Arwert EN, Harney AS, Entenberg D, Wang Y, Sahai E, Pollard JW, Condeelis JS. A Unidirectional Transition from Migratory to Perivascular Macrophage Is Required for Tumor Cell Intravasation. Cell Rep. 2018;23(5):1239-48. doi: 10.1016/j.celrep.2018.04.007. PubMed PMID: 29719241; PMCID: 5946803.