Stable Isotope & Metabolomics Core

Module #1

180 Metabolites 

The targeted metabolomics approach in this assay is based on measurements with the AbsoluteIDQ p180 kit (BIOCRATES Life Sciences AG, Innsbruck, Austria). This method allows simultaneous quantification of 188 metabolites in plasma, tissues or cell pellets using liquid chromatography and flow injection analysis–mass spectrometry. For examples of this module’s utility for Core customers, see:

Walters, R. O., Arias, E., Diaz, A., Burgos, E. S., Guan, F., Tiano, S., Mao, K., Green, C. L., Qiu, Y., Shah, H., Wang, D., Hudgins, A. D., Tabrizian, T., Tosti, V., Shechter, D., Fontana, L., Kurland, I. J., Barzilai, N., Cuervo, A. M., Promislow, D. E. L. & Huffman, D. M. (2018) Sarcosine Is Uniquely Modulated by Aging and Dietary Restriction in Rodents and Humans, Cell reports. 25, 663-676 e6.

Moon, J. Y., Zolnik, C. P., Wang, Z., Qiu, Y., Usyk, M., Wang, T., Kizer, J. R., Landay, A. L., Kurland, I. J., Anastos, K., Kaplan, R. C., Burk, R. D. & Qi, Q. (2018) Gut microbiota and plasma metabolites associated with diabetes in women with, or at high risk for, HIV infection, EBioMedicine. 37, 392-400.

Bonhoure, N., Byrnes, A., Moir, R. D., Hodroj, W., Preitner, F., Praz, V., Marcelin, G., Chua, S. C., Jr., Martinez-Lopez, N., Singh, R., Moullan, N., Auwerx, J., Willemin, G., Shah, H., Hartil, K., Vaitheesvaran, B., Kurland, I., Hernandez, N. & Willis, I. M. (2015) Loss of the RNA polymerase III repressor MAF1 confers obesity resistance, Genes Dev. 29, 934-47.

Mera, P., Laue, K., Ferron, M., Confavreux, C., Wei, J., Galan-Diez, M., Lacampagne, A., Mitchell, S. J., Mattison, J. A., Chen, Y., Bacchetta, J., Szulc, P., Kitsis, R. N., de Cabo, R., Friedman, R. A., Torsitano, C., McGraw, T. E., Puchowicz, M., Kurland, I. & Karsenty, G. (2017) Osteocalcin Signaling in Myofibers Is Necessary and Sufficient for Optimum Adaptation to Exercise, Cell metabolism. 25, 218.

For full list of metabolites, please click here.

Biochemical Name Pubchem ID Super Pathway Sub Pathway
Alanine 5950  Amino acids Alanine metabolism,Aspartate metabolism 
Arginine 3362  Amino acids Urea cycle, Arginine and Proline metabolism 
Asparagine 6267  Amino acids Aspartate metabolism 
Aspartic acid 5960  Amino acids Aspartate metabolism 
Citrulline 9750  Amino acids Urea cycle 
Glutamine 5961  Amino acids Glutamate metabolism 
Glutamic Acid 33032  Amino acids Glutamate metabolism 
Glycine 750  Amino acids Glycine and serine metabolism 
Histidine 6274  Amino acids Histidine metabolism 
Isoleucine 6306  Amino acids Valine, Leucine and Isoleucine Degradation 
Leucine  6106  Amino acids Valine, Leucine and Isoleucine Degradation 
Lysine 5962  Amino acids Lysine Degradation 
Methionine 6137  Amino acids Methionine metabolism 
Ornithine 6262  Amino acids Urea cycle, Arginine and Proline metabolism 
Phenylalanine 6140  Amino acids Phenylalanine and Tyrosine metabolism 
Proline 145742  Amino acids Arginine and Proline metabolism 
Serine 5951  Amino acids Glycine and serine metabolism 
Threonine 6288  Amino acids Threonine and 2-oxobutanoate degradation 
Tryptophan 6305  Amino acids Tryptophan metabolism 
Tyrosine 6057  Amino acids Phenylalanine and Tyrosine metabolism 
Valine 6287  Amino acids Valine,Leucine and Isoleucine metabolism 
Acetylornithine 439232  Amino acids Arginine and proline metabolism 
Asymmetric dimetylarginine 123831  Amino acids Not Available
alpha-Aminoadipic acid 469  Amino acids Lysine Degradation 
Carnosine 439224  Amino acids  beta-Alanine metabolism, Histidine metabolism 
Creatinine 588  Amino acids Creatinine 
Dopa 6047  Amino acids Catecholamine Biosynthesis 
Dopamine 681  Amino acids Catecholamine biosynthesis 
Histamine 774  Amino acids Histidine metabolism 
Kynurenine 161166  Amino acids Tryptophan Degradation,Lysine Degradation 
Methioninesulfoxide 847  Amino acids Methionine metabolism 
Nitrotyrosine 65124  Amino acids Phenylalanine and Tyrosine metabolism 
Hydroxyproline 5810  Amino acids Arginine and Proline metabolism 
Phenylethylamine 1001  Amino acids Tyrosine metabolism 
Putrescine 1045  Amino acids Spermidine and Spermine biosynthesis 
Sacrcosine 1088  Amino acids Glycine and Serine metabolism 
Symmetric dimethylarginine 169148  Amino acids Urea cycle, Arginine and Proline metabolism 
Serotonin 5202  Amino acids Tryptophan metabolism 
Spermidine 1102  Amino acids Spermidine and Spermine biosynthesis 
Spermine 1103  Amino acids Spermidine and Spermine biosynthesis 
Taurine 1123  Amino acids Taurine and Hypotaurine metabolism 
Total dimethylarginine 123831  Amino acids Arginine and Proline metabolism 
Acylcarnitines
C0 to C18: 2 		 40 species Lipid Fatty acid metabolism 
Glycerophospholipids
C14:0 to C44: 6 		 90 species Lipid metabolism Phospholipid biosynthesis 
Sphingolipids
SM C14:1 to SM C26:1 		 15 species Lipid metabolism Sphingolipid biosynthesis 
Hexose  5793  Carbohydrate Glycolysis pathway, Pentose Phosphate pathway , Gluconeogenesis 
 

Module 2 > 

Department Office

Irwin Kurland, M.D.
Director
Room 374, Price Center/Block Research Pavilion

 

Yunping Qiu. Ph.D.
Operations Manager
Room 368, Price Center/Block Research Pavilion

 

Min Cai
Mass Spectrometer Operator
Room 368, Price Center/Block Research Pavilion

 

Xueliang Du, Ph.D.
Seahorse Technician
Room 529, Forchheimer

 
 

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Mass Spectrometer: xueqing.zhao@einsteinmed.org 

Seahorse: xueliang.du@einsteinmed.org