Quantification of ~200 metabolites using LC/MS/MS and untargeted GC/TOF-MS
This assay combines Module 7 with Module 10, and includes quantitation of 20 amino acids, 40+ acyl carnitines, ~100 glycerophospholipids and sphingolipids. For examples of this module’s utility for Core customers, see:
Mera, P., Laue, K., Ferron, M., Confavreux, C., Wei, J., Galan-Diez, M., Lacampagne, A., Mitchell, S. J., Mattison, J. A., Chen, Y., Bacchetta, J., Szulc, P., Kitsis, R. N., de Cabo, R., Friedman, R. A., Torsitano, C., McGraw, T. E., Puchowicz, M., Kurland, I. & Karsenty, G. (2017) Osteocalcin Signaling in Myofibers Is Necessary and Sufficient for Optimum Adaptation to Exercise, Cell metabolism. 25, 218.
| Biochemical Name |
Pubchem ID |
Super Pathway |
SubPathway |
| Untargeted GC/MS analysis using methyl chloroformate derivatization |
|
|
see text |
| Acylcarnitines
C0 to C18:2
|
|
Lipid metabolism |
Fatty acid metabolism |
| Glycerophospholipids
C6:0 to C44:6
|
92 species |
Lipid metabolism |
Phospholipid biosynthesis |
| Sphingolipids
SM C14:1 to SM C26:1
|
15 species |
Lipid metabolism |
Sphingolipid biosynthesis |
Module #10A. Quantification of 40+ acyl carnitines and ~100 phospholipids by LC/MS/MS
This emulates the lipidomic part only of the Biocrates p180 kit.
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