Hepatic recycling deuterated glucose tolerance test (HR-dGTT):
[2-2H]- and [6,6-2H2]glucose are administered as a 1:1 mixture, disappearance of the two isotopes [2-2H]- and [6,6-2H2]glucose is determined by GC/MS. The difference between the two disappearance rates has long been recognized as the standard measure of glucose/glucose-6-P futile cycling. For examples of this module’s utility for Core customers, see:
Vaitheesvaran, B., Yang, L., Hartil, K., Glaser, S., Yazulla, S., Bruce, J. E. & Kurland, I. J. Peripheral effects of FAAH deficiency on fuel and energy homeostasis: role of dysregulated lysine acetylation, PLoS One. 7, e33717.
Haeusler, R. A., Hartil, K., Vaitheesvaran, B., Arrieta-Cruz, I., Knight, C. M., Cook, J. R., Kammoun, H. L., Febbraio, M. A., Gutierrez-Juarez, R., Kurland, I. J. & Accili, D. (2014) Integrated control of hepatic lipogenesis versus glucose production requires FoxO transcription factors, Nature communications. 5, 5190.
Vaitheesvaran, B., Chueh, F. Y., Xu, J., Trujillo, C., Saad, M. F., Lee, W. N., McGuinness, O. P. & Kurland, I. J. (2010) Advantages of dynamic "closed loop" stable isotope flux phenotyping over static "open loop" clamps in detecting silent genetic and dietary phenotypes, Metabolomics. 6, 180-190.
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