Widely Targeted Lipidomic Profiling (WTLP) using the Sciex 6500+ QTRAP
Our newly developed widely targeted lipidomic profiling (WTLP) method employs a MRM based fragmentation method for the Sciex 6500+, combined with Avanti SPLASH© and Lipidyzer© standards, to design a WTLP method for detection and quantitation of ~1300 lipid species in 26 lipid classes, including Ceramides (CER), Cholesterol (CHOL), Cholesterol ester (CE), diacylglycerol (DAG), Triacylglycerol (TAG), dihydroceramides (DCER), free fatty acids (FFA), hexosylceramides (HCER), lactosylceramide (LCER), phosphatidylcholine (PC), phosphatidylethanolamine (PE), lyso-PC (LPC), lyso-PE (LPE), sphingomyelin (SM), phosphatidic acid (PA), phosphatidylinositol (PI), phosphatidylglycerol (PG) and phosphatidylserine (PS). In human plasma, 600-700 lipid species have a coefficient of variation (CV) <30%
Table 1. List of lipids found by our LC/MS/MS MRM method, the number of species for each class, and the ESI polarity.
For an Excel file with the full list of lipids, click here.
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