Tenofovir quantification
Tenofovir (or 9-[(R)-2-(phosphonomethoxy)-propyl]adenine) is a antiretroviral commonly used in human immunodeficiency virus type 1 (HIV-1) treatment. Since accurate analysis of its concentration in biofluids is important for research studies, we developed a sensitive and accurately method the quantify Tenofovir in plasma or urine samples.
Our assay uses Tenofovir_d6 as internal standard. The limit of detection (LOD) is about 0.1 ng/mL, and lower limit of quantification (LLOQ) is 0.3ng/mL. As shown in the following figure, linearity is demonstrated between 0.3ng/mL to 1000ng/mL, with the Tenofovir_d6 standard spiked into urine samples.
Module 14 consists of --- metabolites when 1 C18 column is used (standard for biolipids), and an additional – metabolites can be assessed if the 2nd column option is chosen (pHILIC).
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