Lipogenesis by Deuterated Water
Deuterated water (2H2O) is a versatile reagent which we have used for assessment of de novo lipogenesis/cholesterol synthesis rates using GC/MS. For examples of this module’s utility for Core customers, see:
Jatkar, A., Kurland, I. J. & Judex, S. (2017) Diets High in Fat or Fructose Differentially Modulate Bone Health and Lipid Metabolism, Calcified tissue international. 100, 20-28.
Laurent, G., German, N. J., Saha, A. K., de Boer, V. C., Davies, M., Koves, T. R., Dephoure, N., Fischer, F., Boanca, G., Vaitheesvaran, B., Lovitch, S. B., Sharpe, A. H., Kurland, I. J., Steegborn, C., Gygi, S. P., Muoio, D. M., Ruderman, N. B. & Haigis, M. C. (2013) SIRT4 coordinates the balance between lipid synthesis and catabolism by repressing malonyl CoA decarboxylase, Molecular cell. 50, 686-98.
Haas, J. T., Miao, J., Chanda, D., Wang, Y., Zhao, E., Haas, M. E., Hirschey, M., Vaitheesvaran, B., Farese, R. V., Jr., Kurland, I. J., Graham, M., Crooke, R., Foufelle, F. & Biddinger, S. B. (2012) Hepatic insulin signaling is required for obesity-dependent expression of SREBP-1c mRNA but not for feeding-dependent expression, Cell metabolism. 15, 873-84.
Miao, J., Haas, J. T., Manthena, P., Wang, Y., Zhao, E., Vaitheesvaran, B., Kurland, I. J. & Biddinger, S. B. (2014) Hepatic insulin receptor deficiency impairs the SREBP-2 response to feeding and statins, Journal of lipid research. 55, 659-67.
Zhao, X., Feng, D., Wang, Q., Abdulla, A., Xie, X. J., Zhou, J., Sun, Y., Yang, E. S., Liu, L. P., Vaitheesvaran, B., Bridges, L., Kurland, I. J., Strich, R., Ni, J. Q., Wang, C., Ericsson, J., Pessin, J. E., Ji, J. Y. & Yang, F. (2012) Regulation of lipogenesis by cyclin-dependent kinase 8-mediated control of SREBP-1, The Journal of clinical investigation. 122, 2417-27.
Bonhoure, N., Byrnes, A., Moir, R. D., Hodroj, W., Preitner, F., Praz, V., Marcelin, G., Chua, S. C., Jr., Martinez-Lopez, N., Singh, R., Moullan, N., Auwerx, J., Willemin, G., Shah, H., Hartil, K., Vaitheesvaran, B., Kurland, I., Hernandez, N. & Willis, I. M. (2015) Loss of the RNA polymerase III repressor MAF1 confers obesity resistance, Genes Dev. 29, 934-47.
Haeusler, R. A., Hartil, K., Vaitheesvaran, B., Arrieta-Cruz, I., Knight, C. M., Cook, J. R., Kammoun, H. L., Febbraio, M. A., Gutierrez-Juarez, R., Kurland, I. J. & Accili, D. (2014) Integrated control of hepatic lipogenesis versus glucose production requires FoxO transcription factors, Nature communications. 5, 5190.
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