8002 Quantitative Imaging of Cells

COURSE DESCRIPTION:
The class Quantitative Imaging of Cells is given as interactive lectures with weekly lab sessions and will introduce students to the physical concepts of optical and electron microscopy and their practical applications to biomedical research. Lectures will cover the properties of light, hardware components and applications of standard and advanced microscopes, such as basic light and fluorescence, confocal, TIRF, light-sheet, multiphoton, and superresolution. There will be additional focus on image processing and image presentation, as well as specialty applications including an overview of fluorescent molecules and photomanipulation techniques, FRET, and Optical Tweezers. Subsequent lectures will cover transmission, scanning and cryo electron microscopy. Lab sessions will include hands-on demonstration sessions on microscopes.

COURSE OBJECTIVES:
To give students and overview of modern light and electron microscopy technology and how to apply it to biomedical research.

PREREQUISITES:
None

REQUIRED MATERIALS:
None

SUITABLE FOR 1ST YEAR STUDENTS:
Yes

STUDENT ASSESSMENTS:
3 exams spaced throughout the Block and a final presentation, each 25% of the overall grade, passing level at 80% overall

CREDIT HOURS: 5.0