Daniel Zamith Miranda, Ph.D.

Research Assistant Professor, Department of Medicine (Infectious Diseases)

Area of research

Microbiology, Immunology, Mycology

daniel.zamithmiranda@einsteinmed.edu

Phone

718-430-3659

Location

Albert Einstein College of Medicine Jack and Pearl Resnick Campus 1300 Morris Park Avenue Ullmann Building 107 Bronx, NY 10461

Lab of Daniel Zamith Miranda

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Research Profiles

Professional Interests

In the Nosanchuk Lab we study a variety of pathogenic fungi and their interactions with the host. We are interested in the mechanisms by which pathogenic fungi cause disease, and how the host reacts when challenged with them. Among the disease-promoting mechanisms employed by fungi, we have a particular interest in extracellular vesicles (EVs) released by these cells, and how they impact the immune response. Our experience shows that the release of EVs by each fungal organism elicits a distinct response in the host that range from stimulation to suppression.

Selected Publications

1.	Extracellular Vesicles Regulate Biofilm Formation and Yeast-to-Hypha Differentiation in Candida albicans. Honorato L, de Araujo JFD, Ellis CC, Piffer AC, Pereira Y, Frases S, de Sousa Araújo GR, Pontes B, Mendes MT, Pereira MD, Guimarães AJ, da Silva NM, Vargas G, Joffe L, Del Poeta M, Nosanchuk JD, Zamith-Miranda D, Dos Reis FCG, de Oliveira HC, Rodrigues ML, de Toledo Martins S, Alves LR, Almeida IC, Nimrichter L. mBio. 2022 Jun 28;13(3):e0030122. doi: 10.1128/mbio.00301-22. Epub 2022 Apr 14. PMID: 35420476 Free PMC article.
2.	Methamphetamine Enhances Cryptococcus neoformans Melanization, Antifungal Resistance, and Pathogenesis in a Murine Model of Drug Administration and Systemic Infection. Erives VH, Munzen ME, Zamith-Miranda D, Hernandez H, Manepalli S, Nguyen LN, Hamed MF, Nosanchuk JD, Martinez LR. Infect Immun. 2022 Apr 21;90(4):e0009122. doi: 10.1128/iai.00091-22. Epub 2022 Mar 31. PMID: 35357221
3.	Replicative Aging Remodels the Cell Wall and Is Associated with Increased Intracellular Trafficking in Human Pathogenic Yeasts. Silva VKA, Bhattacharya S, Oliveira NK, Savitt AG, Zamith-Miranda D, Nosanchuk JD, Fries BC. mBio. 2022 Feb 15;13(1):e0019022. doi: 10.1128/mbio.00190-22. Online ahead of print. PMID: 35164553 Free PMC article.
4.	Isolation of Extracellular Vesicles from Candida auris. Zamith-Miranda D, Alves LR, Rodrigues ML, Nimrichter L, Nosanchuk JD. Methods Mol Biol. 2022;2517:173-178. doi: 10.1007/978-1-0716-2417-3_13. PMID: 35674953
5.	Cellular and Extracellular Vesicle RNA Analysis in the Global Threat Fungus Candida auris. Munhoz da Rocha IF, Martins ST, Amatuzzi RF, Zamith-Miranda D, Nosanchuk JD, Rodrigues ML, Alves LR. Microbiol Spectr. 2021 Dec 22;9(3):e0153821. doi: 10.1128/Spectrum.01538-21. Epub 2021 Dec 15. PMID: 34908466 Free PMC article.
6.	A Histoplasma capsulatum Lipid Metabolic Map Identifies Antifungal Targets. Zamith-Miranda D, Heyman HM, Burnet MC, Couvillion SP, Zheng X, Munoz N, Nelson WC, Kyle JE, Zink EM, Weitz KK, Bloodsworth KJ, Clair G, Zucker JD, Teuton JR, Payne SH, Kim YM, Reyes Gil M, Baker ES, Bredeweg EL, Nosanchuk JD, Nakayasu ES. mBio. 2021 Dec 21;12(6):e0297221. doi: 10.1128/mBio.02972-21. Epub 2021 Nov 23. PMID: 34809453 Free PMC article.
7.	Host cell membrane microdomains and fungal infection. Souza TN, Valdez AF, Rizzo J, Zamith-Miranda D, Guimarães AJ, Nosanchuk JD, Nimrichter L. Cell Microbiol. 2021 Dec;23(12):e13385. doi: 10.1111/cmi.13385. Epub 2021 Aug 24. PMID: 34392593 Review.
8.	Transcriptional and translational landscape of Candida auris in response to caspofungin. Zamith-Miranda D, Amatuzzi RF, Munhoz da Rocha IF, Martins ST, Lucena ACR, Vieira AZ, Trentin G, Almeida F, Rodrigues ML, Nakayasu ES, Nosanchuk JD, Alves LR. Comput Struct Biotechnol J. 2021 Sep 14;19:5264-5277. doi: 10.1016/j.csbj.2021.09.007. eCollection 2021. PMID: 34630944 Free PMC article.
9.	Comparative Molecular and Immunoregulatory Analysis of Extracellular Vesicles from Candida albicans and Candida auris. Zamith-Miranda D, Heyman HM, Couvillion SP, Cordero RJB, Rodrigues ML, Nimrichter L, Casadevall A, Amatuzzi RF, Alves LR, Nakayasu ES, Nosanchuk JD. mSystems. 2021 Aug 31;6(4):e0082221. doi: 10.1128/mSystems.00822-21. Epub 2021 Aug 24. PMID: 34427507 Free PMC article.
10.	Omics Approaches for Understanding Biogenesis, Composition and Functions of Fungal Extracellular Vesicles. Zamith-Miranda D, Peres da Silva R, Couvillion SP, Bredeweg EL, Burnet MC, Coelho C, Camacho E, Nimrichter L, Puccia R, Almeida IC, Casadevall A, Rodrigues ML, Alves LR, Nosanchuk JD, Nakayasu ES. Front Genet. 2021 May 3;12:648524. doi: 10.3389/fgene.2021.648524. eCollection 2021. PMID: 34012462 Free PMC article. Review.
11.	Fungal Melanin and the Mammalian Immune System. Liu S, Youngchim S, Zamith-Miranda D, Nosanchuk JD. J Fungi (Basel). 2021 Mar 31;7(4):264. doi: 10.3390/jof7040264. PMID: 33807336 Free PMC article. Review.
12.	Complex and Controversial Roles of Eicosanoids in Fungal Pathogenesis. Mendoza SR, Zamith-Miranda D, Takács T, Gacser A, Nosanchuk JD, Guimarães AJ. J Fungi (Basel). 2021 Mar 28;7(4):254. doi: 10.3390/jof7040254. PMID: 33800694 Free PMC article. Review.
13.	Lessons Learned from Studying Histoplasma capsulatum Extracellular Vesicles. Zamith-Miranda D, Alves LR, Nakayasu ES, Nosanchuk JD. Curr Top Microbiol Immunol. 2021;432:13-18. doi: 10.1007/978-3-030-83391-6_2. PMID: 34972874
14.	Nutritional Conditions Modulate C. neoformans Extracellular Vesicles' Capacity to Elicit Host Immune Response. Marina CL, Bürgel PH, Agostinho DP, Zamith-Miranda D, Las-Casas LO, Tavares AH, Nosanchuk JD, Bocca AL. Microorganisms. 2020 Nov 18;8(11):1815. doi: 10.3390/microorganisms8111815. PMID: 33217920 Free PMC article.
15.	Remodeling of the Histoplasma Capsulatum Membrane Induced by Monoclonal Antibodies. Burnet MC, Zamith-Miranda D, Heyman HM, Weitz KK, Bredeweg EL, Nosanchuk JD, Nakayasu ES. Vaccines (Basel). 2020 Jun 2;8(2):269. doi: 10.3390/vaccines8020269. PMID: 32498228 Free PMC article.
16.	Multi-omics Signature of Candida auris, an Emerging and Multidrug-Resistant Pathogen. Zamith-Miranda D, Heyman HM, Cleare LG, Couvillion SP, Clair GC, Bredeweg EL, Gacser A, Nimrichter L, Nakayasu ES, Nosanchuk JD. mSystems. 2019 Jun 11;4(4):e00257-19. doi: 10.1128/mSystems.00257-19. PMID: 31186339 Free PMC article.
17.	Immunization Strategies for the Control of Histoplasmosis. Roth MT, Zamith-Miranda D, Nosanchuk JD. Curr Trop Med Rep. 2019 Jun;6(2):35-41. doi: 10.1007/s40475-019-00172-3. Epub 2019 Mar 26. PMID: 31772912 Free PMC article.
18.	Extracellular Vesicle-Mediated RNA Release in Histoplasma capsulatum. Alves LR, Peres da Silva R, Sanchez DA, Zamith-Miranda D, Rodrigues ML, Goldenberg S, Puccia R, Nosanchuk JD. mSphere. 2019 Mar 27;4(2):e00176-19. doi: 10.1128/mSphere.00176-19. PMID: 30918062 Free PMC article.
19.	Host membrane glycosphingolipids and lipid microdomains facilitate Histoplasma capsulatum internalisation by macrophages. Guimarães AJ, de Cerqueira MD, Zamith-Miranda D, Lopez PH, Rodrigues ML, Pontes B, Viana NB, DeLeon-Rodriguez CM, Rossi DCP, Casadevall A, Gomes AMO, Martinez LR, Schnaar RL, Nosanchuk JD, Nimrichter L. Cell Microbiol. 2019 Mar;21(3):e12976. doi: 10.1111/cmi.12976. Epub 2018 Dec 7. PMID: 30427108 Free PMC article.
20.	Fungal extracellular vesicles: modulating host-pathogen interactions by both the fungus and the host. Zamith-Miranda D, Nimrichter L, Rodrigues ML, Nosanchuk JD. Microbes Infect. 2018 Oct-Nov;20(9-10):501-504. doi: 10.1016/j.micinf.2018.01.011. Epub 2018 Feb 19. PMID: 29471026 Free PMC article. Review.
21.	The allergic response mediated by fire ant venom proteins. Zamith-Miranda D, Fox EGP, Monteiro AP, Gama D, Poublan LE, de Araujo AF, Araujo MFC, Atella GC, Machado EA, Diaz BL. Sci Rep. 2018 Sep 26;8(1):14427. doi: 10.1038/s41598-018-32327-z. PMID: 30258210 Free PMC article.
22.	Concentration-dependent protein loading of extracellular vesicles released by Histoplasma capsulatum after antibody treatment and its modulatory action upon macrophages. Baltazar LM, Zamith-Miranda D, Burnet MC, Choi H, Nimrichter L, Nakayasu ES, Nosanchuk JD. Sci Rep. 2018 May 23;8(1):8065. doi: 10.1038/s41598-018-25665-5. PMID: 29795301 Free PMC article.
23.	The putative flippase Apt1 is required for intracellular membrane architecture and biosynthesis of polysaccharide and lipids in Cryptococcus neoformans. Rizzo J, Colombo AC, Zamith-Miranda D, Silva VKA, Allegood JC, Casadevall A, Del Poeta M, Nosanchuk JD, Kronstad JW, Rodrigues ML. Biochim Biophys Acta Mol Cell Res. 2018 Mar;1865(3):532-541. doi: 10.1016/j.bbamcr.2017.12.007. Epub 2017 Dec 29. PMID: 29291962 Free PMC article.
24.	Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds. de-Souza-Silva CM, Guilhelmelli F, Zamith-Miranda D, de Oliveira MA, Nosanchuk JD, Silva-Pereira I, Albuquerque P. J Vis Exp. 2018 Feb 14;(132):57127. doi: 10.3791/57127. PMID: 29553507 Free PMC article.
25.	Heat Shock Proteins in Histoplasma and Paracoccidioides. Cleare LG, Zamith-Miranda D, Nosanchuk JD. Clin Vaccine Immunol. 2017 Nov 6;24(11):e00221-17. doi: 10.1128/CVI.00221-17. Print 2017 Nov. PMID: 28903987 Free PMC article. Review.
26.	The putative autophagy regulator Atg7 affects the physiology and pathogenic mechanisms of Cryptococcus neoformans. Oliveira DL, Fonseca FL, Zamith-Miranda D, Nimrichter L, Rodrigues J, Pereira MD, Reuwsaat JC, Schrank A, Staats C, Kmetzsch L, Vainstein MH, Rodrigues ML. Future Microbiol. 2016 Oct;11:1405-1419. doi: 10.2217/fmb-2016-0090. Epub 2016 Oct 18. PMID: 27750454
27.	Group V Secretory Phospholipase A2 Is Involved in Tubular Integrity and Sodium Handling in the Kidney. Silva-Filho JL, Peruchetti DB, Moraes-Santos F, Landgraf SS, Silva LS, Sirtoli GM, Zamith-Miranda D, Takiya CM, Pinheiro AA, Diaz BL, Caruso-Neves C. PLoS One. 2016 Jan 28;11(1):e0147785. doi: 10.1371/journal.pone.0147785. eCollection 2016. PMID: 26820468 Free PMC article.
28.	Traveling into Outer Space: Unanswered Questions about Fungal Extracellular Vesicles. Rodrigues ML, Godinho RM, Zamith-Miranda D, Nimrichter L. PLoS Pathog. 2015 Dec 3;11(12):e1005240. doi: 10.1371/journal.ppat.1005240. eCollection 2015 Dec. PMID: 26633018 Free PMC article. Review. No abstract available.
29.	P2×7 purinergic signaling in dilated cardiomyopathy induced by auto-immunity against muscarinic M2 receptors: autoantibody levels, heart functionality and cytokine expression. Martinez CG, Zamith-Miranda D, da Silva MG, Ribeiro KC, Brandão IT, Silva CL, Diaz BL, Bellio M, Persechini PM, Kurtenbach E. Sci Rep. 2015 Nov 23;5:16940. doi: 10.1038/srep16940. PMID: 26592184 Free PMC article.
30.	Probiotic Saccharomyces cerevisiae strains as biotherapeutic tools: is there room for improvement? Palma ML, Zamith-Miranda D, Martins FS, Bozza FA, Nimrichter L, Montero-Lomeli M, Marques ET Jr, Douradinha B. Appl Microbiol Biotechnol. 2015 Aug;99(16):6563-70. doi: 10.1007/s00253-015-6776-x. Epub 2015 Jul 4. PMID: 26142388 Review.
31.	Hypertonic environment elicits cyclooxygenase-2-driven prostaglandin E2 generation by colon cancer cells: role of cytosolic phospholipase A2-alpha and kinase signaling pathways. Gentile LB, Piva B, Capizzani BC, Furlaneto LG, Moreira LS, Zamith-Miranda D, Diaz BL. Prostaglandins Leukot Essent Fatty Acids. 2010 Feb-Mar;82(2-3):131-9. doi: 10.1016/j.plefa.2009.11.005. Epub 2009 Dec 9. PMID: 20004562