Gene Targeting and Transgenic Facility

The Gene Targeting and Transgenic Facility is a centralized facility for the production of transgenic and gene-targeted mice. The facility’s services allow researchers to modify the mouse genome through methods of conventional transgenesis. In addition, the facility is integrated with the Gene Modification facility to generate gene-targeted mice using CRISPR/Cas9 technology. It also offers services for BAC transgenesis and the generation of chimeric mice by injection of embryonic stem cells into blastocysts. Finally, the facility offers a wide variety of reproductive services including in vitro fertilization and mouse line re-derivation, sperm and embryo cryopreservation, and storage of cryopreserved sperm and embryos.

  • Mouse Line Production
    • CRISPR/Cas9-mediated generation of:
      • Constitutive knockout models
      • Knock-in models (small modifications <100bp)
      • Conditional knockout models (Loxp alleles)
      • Complex genetically engineered mouse models
      • Traditional transgenic mice/BAC transgenic mice/lentiviral periviteline injection
      • ES cell blastocyst injection for chimera production mouse line generation
  • Reproductive Services
    • In vitro fertilization
    • Mouse line re-derivation
    • Embryo cryopreservation and storage
    • Sperm cryopreservation and storage
    • Ovary transplantation
  • Breeding/Mouse Colony Maintenance
    • Establishment of mouse colonies
    • Breeding of homozygous mouse lines
    • Genotyping

Related Shared Facilities
Related Shared Facilities

Gene Modification

Transgenic Mouse

Selected Publications


Location and Contacts

Gene Targeting and Transgenic Facility Price Center/Block Research Pavillion B110