Proteostasis of Aging Core Alterations in proteostasis have been described in multiple age-related disorders and are also tightly linked to additional mechanisms of aging such as molecular damage, cellular response to stress, metabolism, inflammation and stem cell functioning. Genetic interventions in invertebrates and in mammals, now support beneficial effects in life- and health-span of modulating components of the intracellular pathways responsible for proteostasis maintenance, chaperones and the proteolytic systems. Translation of these findings into humans requires: 1) characterization of changes in proteostasis in old organisms; 2) understanding the molecular mechanisms behind proteostasis loss in aging and 3) extensive testing of the effect of chemical and natural compounds on the proteostasis machinery and their future development into drugs. The Proteostasis of Aging Core (PAC) was created in response to these needs of the aging research community and, during its ten years in operation, has been providing reliable and highly validated services, state-of-the art methodology, expertise and cutting-edge knowledge related to proteostasis to internal and external E-NSC members and aging research groups worldwide. Personnel Director: Ana Maria Cuervo MD Ph.D. and Simone Sidoli, Ph.D. Operations Manager: Antonio Diaz Consultants: Dr. Evris Gavathiotis, Dr. Susmita Kaushik Services Assays Autophagy profiling LC3-II flux Protein turnover Electron microscopy and morphometry Lysosomal functions Enzymes assays Reagents and Samples Autophagy antibody panel Autophagy reporters Lysosomal markers Consulting Autophagy methods and assays (Dr. Susmita Kaushik) Drug developments and design (Dr. Evris Gavathiotis) Prices PROTEIN TURNOVER Type of Service NSC Member Rate Others Complete Protein Turnover analysis $600 $950 Protein synthesis (4 samples with triplicates) $200 $300 Protein degradation (1 type sample 6 conditions with triplicates) $500 $600 AUTOPHAGY ASSAYS Type of Service NSC Member Rate Others Macroautophagy (MA) LC3 flux - immunoblot (8 conditions) $350 $550 mCherry-GFP-LC3 High content microscopy/96 well plate $600 $800 Mitophagy - immunofluorescence colocalization/8 well $300 $400 Lipophagy - immunofluorescence colocalization/8 well $500 $750 Em morphometric analysis of autophagic compartments*** $200 $300 AUTOPHAGY ASSAYS Type of Service NSC Member Rate Others KFERQ-Dendra - High content microscopy/96 well plate $600 $800 Lysomal Function Lysosomal compartment – Lysotracker/ 8 well $250 $250 Lysosomal markers (panel) - Immunoblot $300 $600 AUTOPHAGY ASSAYS Type of Service NSC Member Rate Others Degron reporter – High content microscopy/ 96 –well plate $650 $950 Consultation Consultation on study design, methods, interpretation $95/hr $200/hr Training in proteostasis analysis procedures $50/hr $-- Other Services Customized analysis of proteostasis Negotiable Negotiable
