The 3DHistech P250 Slide Scanner is an automated high capacity slide scanner (up to 250 slides per batch). It provides brightfield and fluorescence scanning of whole slides. It is a drop-off service.
Attention: The NIH requires that all publications using data from this instrument must list SIG #1S10OD026852-01A1
Objectives:
20X NA=0.8 AIR
40X NA=0.95 AIR
Fluorescence Filters: DAPI, FITC, TRITC, CY5, CY7
Light Source:
Brightfield (immunohistochemistry): stroboscopic illumination with Lumencor Spectra
Fluorescence: Lumencor LED light source
Image Detection:
Brightfield: (immunohistochemistry): high-speed, 4-megapixel, color CMOS camera
Fluorescence: low-noise high-speed 5.5-megapixel CCD camera
Software: Pannoramic Scanner for acquisition. Slide Viewer for visualization. Quant Center for analysis.
Additional Features: Extended focus, Z-stacking; Batch acquisition (250 Slides)
Special Rules for Slides:
- Use hard mounting media only
- No nail polish (or other sealing agent), and no wet mount
- No cover slip protruding over slide edge
- No protruding labels
- Slides need to be clean and dry: clean the back of the slide with ethanol and scrape the edges of the slide with a razor blade
- When circling sample with sharpie do so on the coverglass side
Instructions to Download Viewer Software:
Case Viewer:
- Go to: https://www.3dhistech.com/research/software/software-downloads/
- Click “Download SlideViewer 2.7 for Windows”
Note: This software does not work on Mac or Linux computers.
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