Núcleo de citogenética molecular The Molecular Cytogenetics Core provides tools for the preparation of human and murine samples suitable for molecular genetic and cytogenetic analysis of the entire genome. These tools include the establishment of EBV transformed cell lines; isolation of DNA and mRNA from a variety of tissue culture samples as well as primary biopsies; preparation of metaphase chromosomes suitable for fluorescence in situ hybridization (FISH) and Spectral Karyotyping (SKY) or whole chromosome paints for human and mouse genome. The core personnel is trained to hybridize commercial probes and to designed locus specific probes for regions of interest to investigators. All the probes are custom designed and in house generated. Recursos de shRNA shRNA Libraries Servicios Acceder a los Servicios FISH HYBRIDIZATION: Locus specific probes and chromosome painting (human and mouse) up to three colors SKY-Spectral Karyotyping (human and mouse) DAPI-Staining and Chromosome Count Generation of Locus Specific Probes (BAC clones, human and mouse) EBV transformation of human lymphocytes DNA Isolation mRNA Isolation Tissue Culture PBMC Isolation and enrichment of other blood cells (T,B and eosinophils) DNA and Cell Lines Repository Picogreen DNA quantification Mycoplasma detection RNAi reagents from human and mouse GIPZ and TRC libraries IncuCyte Analyzer Instalaciones compartidas relacionadas Imágenes analíticasHistotechnology and Comparative Pathology Transgenic and Gene Targeting Afiliaciones al centro Montefiore Einstein Comprehensive Cancer Center Publicaciones Seleccionadas Cytogenetic and Genome Research, 2008;121(1):7-9 Human Molecular Genetics, 2008 Sep 15;17(18):2819-33. Journal of Medical Genetics, 2008 Mar 18 Ubicación y contactos Molecular Cytogenetics Core Price Center/Block Research Pavillion 407 Bernice Morrow, Ph.D.Director científico718-678-1121bernice.morrow@einsteinmed.eduJidong Shan, Ph.D.Director de operaciones718-678-1155jidong.shan@einsteinmed.edu
