Biohazard Immunology/Pathology The Biohazard Immunology/Pathology Core provides a centralized facility for performing a range of assays and services that facilitate the functional and anatomical analysis of cells and tissues by Einstein investigators. The services are provided with biosafety containment and with a goal to facilitate understanding of the mechanisms that mediate HIV and opportunistic infection and tissue responses. Services Access the Services Quantification of cytokines, chemokines and other mediators by ELISA, Luminex or flow cytometry. Laser-capture microdissection of infected cells Assistance in characterization and differentiation of cultured cells. Extraction and analysis of RNA from uninfected and infected tissues or cells. In situ hybridization analyses of tissues for microscopic evaluation of cell type-specific expression of cytokines, chemokines and other mediators. Tissue processing and preparation for histopathology analysis of HIV-1 or opportunistic pathogen-infected human clinical samples and animal tissues. Immunocytochemistry for HIV-1 antigens or nucleic acids, other pathogen-specific antigen detections, leukocyte surface markers, cell-specific markers and mediators such as cytokines and chemokines. Interpretation and photographic documentation of the histopathology and immunocytochemistry findings. Consultation and training Related Shared Facilities Analytical Imaging Histotechnology and Comparative Pathology Center Affiliations Einstein-Montefiore Center for AIDS Research Selected Publications Journal of Neurovirology, 2007 Dec;13(6):536-48 Neuropathol Appl Neurobiol. 2011 Aug;37(5):464-83. PLoS One. 2013 Jul 26;8(7):e69270. The Journal of Infectious Diseases, 2008 Jun;197(11):1621-7 Location and Contacts Biohazard Immunology/Pathology Forchheimer 706S; Chanin 624 Joan W Berman, Ph.D.Co-Director718-430-2587joan.berman@einsteinmed.orgSunhee Lee, M.D.Co-Director718-430-2666sunhee.lee@einsteinmed.org
